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Chapters
- 00:05Title
- 01:34Coating Surfaces with Matrix Ligand
- 02:54Preparation of Cells
- 03:50Preparing the Low-intensity Pulsed Ultrasound (LIPUS) Stimulation
- 05:10Focal Adhesion Analysis by Immunofluorescence
- 05:58Biochemical Assays
- 06:24Representative Results
- 08:18Conclusion
Denne protokol beskrives stimuleringen af dyrkede fibroblaster med lav intensitet pulserede ultralyd, som driver fokal adhesionsdannelse og Rac1 aktivering ved at efterligne indgreb af transmembrane matrix-receptor, syndecan-4. Denne fremgangsmåde muliggør undersøgelse af et succesfuldt klinisk teknik på det cellulære niveau, hvorved der tilvejebringes mulighed for forbedring af behandlingen.
Tags
Low-intensity UltrasoundAdhesion-dependent SignalsMulticellular OrganismsCell BehaviorExtracellular MatrixMatrix-engagementCell MigrationCell ProliferationCell SecretionCell DifferentiationMolecular InteractionsExtracellular Matrix ReceptorsIntegrinsMechanical LinkAdhesion-dependent Signaling CascadesTransmembrane ReceptorsSyndecan-4α5β1-integrinFibronectinSyndecan-4 SignalingFibroblast MigrationHealing RetardationFibronectin-dependent SignalingRepair Processes
