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To-og tre-dimensjonale Live-Cell Imaging av DNA Damage Response Proteiner

Article DOI: 10.3791/4251 • 10:24 min • September 28th, 2012
September 28th, 2012 •
1,2, 3, 1,2,4
1Department of Radiation Oncology, Virginia Commonwealth University, 2Department of Biochemistry & Molecular Biology, Virginia Commonwealth University, 3Department of Anatomy & Neurobiology, Virginia Commonwealth University, 4Massey Cancer Center, Virginia Commonwealth University

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Denne protokollen beskriver en fremgangsmåte for å visualisere en DNA dobbel tråd pause signal protein aktiveres i respons til DNA-skade, så vel som dens lokalisering under mitose.

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Genetikk Molecular Biology cellebiologi biokjemi DNA Double-tråd pauser DNA skade respons proteiner live celle bildebehandling 3D celle bildebehandling konfokalmikroskopi
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