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Murine Colitis Modeling using Dextran Sulfate Sodium (DSS)
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Murine Colitis Modeling using Dextran Sulfate Sodium (DSS)

Murine Colitis Modeling using Dextran Sulfate Sodium (DSS)

DOI:
10.3791/1652-v

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08:39 min

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January 19, 2010

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, ,
1Department of Cancer Biology,Vanderbilt University, 2Departments of Medicine and Cancer Biology,Vanderbilt University

Chapters

  • 00:00Title
  • 00:34Introduction
  • 00:52Injury-Repair Colitis Model
  • 02:06Harvesting the Colon
  • 04:04Processing as “Sausages” for Macroscopic Analysis of the Entire Colon
  • 05:52Making “Swiss Rolls for the Histological Analysis of Acute Colitis
  • 07:00Acute Colitis in the Dextran Sulfate Sodium Model
  • 07:59Conclusion

Summary

Automatic Translation

Automatic Translation

Dextran sulfate sodium (DSS) administered in the drinking water is an established murine inflammatory injury model of acute colitis. This protocol outlines the method for DSS treatment and the preparation of tissues.

Tags

Murine Colitis ModelingDextran Sulfate Sodium (DSS)ColitisUlcerative ColitisCrohn’s DiseaseCrohn’s ColitisAbdominal PainDistentionMalabsorptionDiarrheaHematocheziaMucus In StoolEnvironmentGeneticsEpithelial Barrier DysfunctionInflammatory Bowel DiseaseAnimal Models Of ColitisLow-molecular Weight Dextran Sodium Sulfate (DSS)Epithelial DamageInflammatory ResponseAcute InjuryChronic Inflammatory Diseases3% DSS In Drinking WaterWeight LossBloody StoolsDiarrheaEuthanizedNecropsy Dissected ColonsMicroscopic AnalysisMacroscopic Analysis

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