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JoVE Journal Biology
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Adipo-Clear: En vev fjerne metoden for tredimensjonale Imaging av fettvev

Article DOI: 10.3791/58271
July 28th, 2018 •
1, 1, 2,3, 1
1Laboratory of Molecular Metabolism, The Rockefeller University, 2Laboratory of Brain Development and Repair, The Rockefeller University, 3Laboratory of Molecular Genetics, The Rockefeller University

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July 28th, 2018

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På grunn av høy lipid innhold, har fettvev vært utfordrende for å visualisere bruke tradisjonelle histologiske metoder. Adipo-Clear er en vev fjerne teknikk som gjør at robust merking og høy oppløsning volumetriske fluorescerende avbilding av fettvev. Her beskriver vi metodene for eksempel forberedelse, forbehandling, flekker, fjerne og montering for bildebehandling.

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