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Chapters
- 00:05Title
- 01:50Mass Spectrometry Measurement 1: Proton-bound Cluster Ion Formation
- 04:05Mass Spectrometry Measurement 2: the Collosion-induced Dissociation (CID) Bracketing Experiments
- 05:36Mass Spectrometry Measurement 3: the Kinetic Method
- 06:51Data Analysis
- 08:32Results: CID Spectra and Thermo-kinetic Plots
- 09:56Conclusion
Fastsættelsen af gas-fase syrer af cystein-holdige oligopeptider beskrives. Eksperimenterne udføres ved hjælp af en tripel kvadrupol massespektrometer. De relative syrer af peptiderne måles ved hjælp kollision-induceret dissociation eksperimenter, og de kvantitative syrer bestemmes ved hjælp af de udvidede Cooks kinetisk metode.
Tags
Gas-phase AciditiesOligopeptidesAmino Acid ResiduesFolded ProteinsCysteine ResidueN-terminusC-terminusExperimental StudiesCondensed PhaseAqueous SolutionsSolvent EffectsActive SitesInterior RegionIntrinsic Acid-base PropertiesSolvent-free EnvironmentMeasurement MethodCooks Kinetic MethodMass SpectrometerElectrospray Ionization (ESI) Ion Source
