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Determination of the Gas-phase Acidities of Oligopeptides
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JoVE Journal Chemistry
Determination of the Gas-phase Acidities of Oligopeptides

Determination of the Gas-phase Acidities of Oligopeptides

DOI:
10.3791/4348-v

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11:00 min

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June 24, 2013

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, , , ,
1Department of Chemistry,University of the Pacific

Chapters

  • 00:05Title
  • 01:50Mass Spectrometry Measurement 1: Proton-bound Cluster Ion Formation
  • 04:05Mass Spectrometry Measurement 2: the Collosion-induced Dissociation (CID) Bracketing Experiments
  • 05:36Mass Spectrometry Measurement 3: the Kinetic Method
  • 06:51Data Analysis
  • 08:32Results: CID Spectra and Thermo-kinetic Plots
  • 09:56Conclusion

Summary

Automatic Translation

Automatic Translation

The determination of the gas-phase acidities of cysteine-containing oligopeptides is described. The experiments are performed using a triple quadrupole mass spectrometer. The relative acidities of the peptides are measured using collision-induced dissociation experiments, and the quantitative acidities are determined using the extended Cooks kinetic method.

Tags

Gas-phase AciditiesOligopeptidesAmino Acid ResiduesFolded ProteinsCysteine ResidueN-terminusC-terminusExperimental StudiesCondensed PhaseAqueous SolutionsSolvent EffectsActive SitesInterior RegionIntrinsic Acid-base PropertiesSolvent-free EnvironmentMeasurement MethodCooks Kinetic MethodMass SpectrometerElectrospray Ionization (ESI) Ion Source

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