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Determination of the Gas-phase Acidities of Oligopeptides
JoVE Journal
Chemistry
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JoVE Journal Chemistry
Determination of the Gas-phase Acidities of Oligopeptides

Determination of the Gas-phase Acidities of Oligopeptides

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11:00 min

June 24, 2013

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11:00 min
June 24, 2013

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The determination of the gas-phase acidities of cysteine-containing oligopeptides is described. The experiments are performed using a triple quadrupole mass spectrometer. The relative acidities of the peptides are measured using collision-induced dissociation experiments, and the quantitative acidities are determined using the extended Cooks kinetic method.

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