Determination of the Gas-phase Acidities of Oligopeptides

The overall goal of this procedure is to apply the cook's kinetic method to determine the gas phase acidity of ine poly allin peptide. This is accomplished by first generating proton bound cluster ions. The second step is to perform the collision induced dissociation bracketing experiment.

Next, the selected reaction monitoring experiment is performed. The final step is to analyze the data collected from the selected reaction monitoring experiments. Ultimately, the kinetic plot is used to generate the value of the gas phase acidity.