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Isolatie van normale en kankergeassocieerde fibroblasten van verse weefsels door fluorescentie-geactiveerde celsortering (FACS)
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Isolatie van normale en kankergeassocieerde fibroblasten van verse weefsels door fluorescentie-geactiveerde celsortering (FACS)

Article DOI: 10.3791/4425-v • 11:31 min • January 14th, 2013
January 14th, 2013 •
1, 1, 1, 1, 1
1Department of Pathology, Sackler School of Medicine, Tel Aviv University

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Cancer Associated Fibroblasten (CAF) te vergemakkelijken tumorinitiatie, groei en progressie door te geven dat bevordert de proliferatie, angiogenese en ontsteking. Hier beschrijven we een methode om pure populaties van normale fibroblasten en CAF isoleren van verse muis en humane weefsels door celsortering, met PDGFRα als oppervlaktemerker.

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Kankerbiologie Cellular Biology Moleculaire Biologie Geneeskunde Oncologie Pathologie Bioengineering Biomedische Technologie Kanker-geassocieerde fibroblasten fibroblast FACS-sortering PDGFRalpha Borstkanker huid carcinoom stroma tumor kanker weefsel cel cultuur mens muis diermodel
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