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Immunology and Infection

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Real-time Live Imaging af T-celle signalering Complex Formation

Article DOI: 10.3791/50076 • 10:31 min • June 23rd, 2013
June 23rd, 2013 •
*1, *1, 1
1The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University
* These authors contributed equally

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Vi beskriver en live-cell imaging metode, der giver indsigt i protein dynamik under T-celleaktivering proces. Vi viser den kombinerede anvendelse af T-celle-spredning assay konfokal mikroskopi og billedbehandling analyse at give kvantitative resultater til følge signaleringskomplekser dannelse hele T-celle aktivering.

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Immunologi cellebiologi molekylærbiologi medicin T-celle aktivering live-cell imaging Signal transduktion konfokal mikroskopi signalering kompleks Co-lokalisering analyse fluorescens cellebiologi T-celle celle billedbehandling
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