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Real-time Live Imaging af T-celle signalering Complex Formation
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Immunology and Infection
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JoVE Journal Immunology and Infection
Real-time Live Imaging of T-cell Signaling Complex Formation

Real-time Live Imaging af T-celle signalering Complex Formation

DOI:
10.3791/50076-v

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10:31 min

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June 23, 2013

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, ,
1The Mina and Everard Goodman Faculty of Life Sciences,Bar-Ilan University

Chapters

  • 00:05Title
  • 01:28T-cell Spreading Assay Preparation
  • 02:52T-cell Activation and Imaging
  • 07:21Imaging Data Analysis
  • 08:42Results: Live Imaging of SLP76 Deficient T-cells
  • 09:48Conclusion

Summary

Automatic Translation

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Vi beskriver en live-cell imaging metode, der giver indsigt i protein dynamik under T-celleaktivering proces. Vi viser den kombinerede anvendelse af T-celle-spredning assay konfokal mikroskopi og billedbehandling analyse at give kvantitative resultater til følge signaleringskomplekser dannelse hele T-celle aktivering.

Tags

T-cell SignalingImmune SystemT LymphocytesT-cell ActivationT-cell Antigen Receptor (TCR)Antigen Presenting Cells (APCs)Immune SynapseProtein-protein InteractionsLive Cell ImagingProtein Complex FormationReal-time ImagingJurkat CellsFluorescent Proteins

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