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Journal
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Immunology and Infection
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T细胞实时成像实时信号复合物的形成
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Real-time Live Imaging of T-cell Signaling Complex Formation
Please note that all translations are automatically generated.
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T细胞实时成像实时信号复合物的形成
DOI:
10.3791/50076-v
•
10:31 min
•
June 23, 2013
•
Elad Noy*
1
,
Maor H. Pauker*
1
,
Mira Barda-Saad
1
The Mina and Everard Goodman Faculty of Life Sciences
,
Bar-Ilan University
Chapters
00:05
Title
01:28
T-cell Spreading Assay Preparation
02:52
T-cell Activation and Imaging
07:21
Imaging Data Analysis
08:42
Results: Live Imaging of SLP76 Deficient T-cells
09:48
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述一个活细胞成像的方法在T细胞活化过程中,提供了洞察蛋白质动力学。我们展示了结合使用的T细胞扩散法,激光共聚焦显微镜成像分析得到的定量结果,按照整个T细胞活化信号复合物的形成。
Tags
T-cell Signaling
Immune System
T Lymphocytes
T-cell Activation
T-cell Antigen Receptor (TCR)
Antigen Presenting Cells (APCs)
Immune Synapse
Protein-protein Interactions
Live Cell Imaging
Protein Complex Formation
Real-time Imaging
Jurkat Cells
Fluorescent Proteins
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