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Chapters
- 00:05Title
- 01:48Assembling the Chemostat Vessels
- 03:16Preparing the Media
- 04:06Calibrating Dissolved Oxygen Probes and Setting Up Chemostat
- 06:22Acquiring Cell Samples Growing in Steady-state Conditions
- 08:11Analysis of Growth Rate Differences between Genotypes in Controlled Environments Using Flow Cytometry-based Competition Analysis
- 09:41Experimental Evolution
- 10:50Representative Results
- 12:31Conclusion
Cell growth rate is a regulated process and a primary determinant of cell physiology. Continuous culturing using chemostats enables extrinsic control of cell growth rate by nutrient limitation facilitating the study of molecular networks that control cell growth and how those networks evolve to optimize cell growth.
