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Brug af kemostater i Mikrobiel Systembiologi
JoVE Journal
Biology
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JoVE Journal Biology
The Use of Chemostats in Microbial Systems Biology
DOI:

13:19 min

October 14, 2013

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Chapters

  • 00:05Title
  • 01:48Assembling the Chemostat Vessels
  • 03:16Preparing the Media
  • 04:06Calibrating Dissolved Oxygen Probes and Setting Up Chemostat
  • 06:22Acquiring Cell Samples Growing in Steady-state Conditions
  • 08:11Analysis of Growth Rate Differences between Genotypes in Controlled Environments Using Flow Cytometry-based Competition Analysis
  • 09:41Experimental Evolution
  • 10:50Representative Results
  • 12:31Conclusion

Summary

Automatic Translation

Cell vækstrate er en reguleret proces, og en primær faktor for celle fysiologi. Kontinuerlig dyrkning ved hjælp kemostater muliggør ydre kontrol af cellevækst sats ved næringssaltbegrænsning lette studiet af molekylære netværk, der styrer cellevækst og hvordan disse net udvikler sig til at optimere cellevækst.

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