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Meting van vacuolaire pH en Cytosolic<em> In Vivo</em> In de gistcel Schorsingen
JoVE Journal
Biology
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JoVE Journal Biology
Measurement of Vacuolar and Cytosolic pH In Vivo in Yeast Cell Suspensions
DOI:

13:55 min

April 19, 2013

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Chapters

  • 00:05Title
  • 02:12Measurement of Vacuolar pH In Vivo using BCECF-AM
  • 07:17Measurement of Cytosolic pH In Vivo using Yeast pHluorin
  • 11:54Results: Vacuolar and Cytosolic pH Changes in Response to Glucose and Other Stimuli
  • 13:25Conclusion

Summary

Automatic Translation

Vacuolaire en cytosolische pH kan worden gemeten in levende gist (<em> S. cerevisiae</em>) Cellen middels ratiometrisch fluorescente kleurstoffen gelokaliseerd in specifieke cellulaire compartimenten. We beschrijven de procedures voor het meten van pH van de vacuole met BCECF-AM, die lokaliseert aan de vacuole in gist, en cytosolische pH met een cytosolisch ratiometrische pH-gevoelige GFP (gist pHluorin).

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