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Oprensning og microRNA profilering af Exosomer basis af blod og Kultur Medier
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Oprensning og microRNA profilering af Exosomer basis af blod og Kultur Medier

Article DOI: 10.3791/50294-v • 10:45 min • June 14th, 2013
June 14th, 2013 •
1, 1, 1
1Department of Pharmacology & Physiology, Drexel University College of Medicine

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Tilstedeværelsen af ​​stabile microRNA (miRNA) i exosomes har skabt enorm interesse som en ny form for intercellulær kommunikation, for deres potentielle anvendelighed som biomarkører og som en vej til terapeutisk intervention. Her viser vi exosome oprensning fra blod og kultur medier efterfulgt af kvantitativ PCR til at identificere miRNA, der transporteres.

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Genetik Molekylærbiologisk Institut cellebiologi medicin Genomics Farmakologi Hematology Exosomer RNA MicroRNA biomarkører farmakologiske Exosomer microRNA qPCR PCR blod biomarkør TLDA profilering sekventering cellekultur
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