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En metode for mus bukspyttkjertelens Isolering og Intracellulær cAMP Bestemmelse
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En metode for mus bukspyttkjertelens Isolering og Intracellulær cAMP Bestemmelse

Article DOI: 10.3791/50374-v • 12:33 min • June 25th, 2014
June 25th, 2014 •
1, 2, 3, 2
1Department of Nutrional Sciences, University of Wisconsin-Madison, 2Department of Medicine, Division of Endocrinology, Diabetes, and Metabolism, University of Wisconsin-Madison, 3School of Pharmacy, University of Waterloo

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Analysere in vitro β-cellefunksjon ved hjelp av isolerte mus Langerhanske øyer er en viktig komponent i studiet av diabetes patofysiologi og behandlingsformer. Mens mange nedstrøms applikasjoner som er tilgjengelige, denne protokollen beskriver spesielt måling av intracellulær cyklisk adenosin monofosfat (cAMP) som en viktig parameter å bestemme β-cellefunksjon.

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Fysiologi holme isolasjon insulinsekresjon β-celle diabetes cAMP produksjon mus
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