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活细胞成像早期自噬活动:Omegasomes与超越
JoVE Journal
Biology
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JoVE Journal Biology
Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond
DOI:

09:00 min

July 27, 2013

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Chapters

  • 00:05Title
  • 01:17Preparing Cells
  • 02:59Preparing the Incubation Chamber for Live Cell Imaging
  • 04:04Microscopy
  • 05:22Results: LC3-positive Autophagosomes Originate from DFCP1-positive Omegasomes
  • 08:28Conclusion

Summary

Automatic Translation

时间推移显微镜荧光标记的细胞自噬标记允许自噬反应具有高时空分辨率的动态监测。使用特定的自噬和细胞器标记在3种不同颜色的组合,我们可以按照自噬体形成在一个强大的空间和时间上下文的蛋白质的贡献。

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