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Chapters
- 00:05Title
- 01:25Fluorescent Dextran Coating of Glass
- 02:46Fluorescent Actin Filaments on Glass
- 03:27Microsphere Fluorescent Beads and Fiducial Markers
- 03:54Epidermal Growth Factor Cell Staining
- 04:56Microscopy Acquisition of STORM Data Using Alexa 647 Dye
- 06:28Reconstructing Super-resolution Images from Raw Data Using rainSTORM
- 08:39Box Tracking and Drift Correction Using rainSTORM
- 09:18Results: STORM Microscopy Assessed Using Test Samples
- 16:26Conclusion
We describe the preparation of three test samples and how they can be used to optimize and assess the performance of STORM microscopes. Using these examples we show how to acquire raw data and then process it to acquire super-resolution images in cells of approximately 30-50 nm resolution.
