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JoVE Journal
Biology

This content is Open Access.

Test Samples for Optimizing STORM Super-Resolution Microscopy
 

Test Samples for Optimizing STORM Super-Resolution Microscopy

Article DOI: 10.3791/50579
September 6th, 2013

Chapters

Summary September 6th, 2013

We describe the preparation of three test samples and how they can be used to optimize and assess the performance of STORM microscopes. Using these examples we show how to acquire raw data and then process it to acquire super-resolution images in cells of approximately 30-50 nm resolution.

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