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JoVE Journal Chemistry
Multiplexed Fluorescent Microarray for Human spytprotein analyse ved hjælp polymermikrosfærer og fiber-optisk Bundter
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Multiplexed Fluorescent Microarray for Human spytprotein analyse ved hjælp polymermikrosfærer og fiber-optisk Bundter

Article DOI: 10.3791/50726-v • 08:50 min • October 10th, 2013
October 10th, 2013 •
1, 1,2, 1, 3, 1
1Department of Chemistry, Tufts University, 2Department of Analytical Chemistry, Complutense University (Spain), 3Department of Electrical and Computer Engineering, Tufts University

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Vi beskriver en procedure for profilering spytproteiner hjælp multiplexede mikrosfære-baserede antistof arrays. Monoklonale antistoffer blev kovalent bundet til fluorescerende farvestof-kodet 4,5 um polymermikrokugler hjælp carbodiimidkemi. De modificerede mikrosfærer blev deponeret i fiberoptiske microwells'ne måle protein niveauer i spyt ved hjælp af fluorescens sandwichimmunassays.

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Kemi protein sensing microarray multiplex fluorescerende kvantificering fiberoptisk biosensor mikrosfære-baserede immunanalyser spyt analyse microsphere kodning
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