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复用荧光微阵列人类唾液蛋白质分析用高分子微球和光纤束
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JoVE Journal Chemistry
Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles
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复用荧光微阵列人类唾液蛋白质分析用高分子微球和光纤束

DOI:
10.3791/50726-v

•

08:50 min

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October 10, 2013

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, , , ,
1Department of Chemistry,Tufts University, 2Department of Analytical Chemistry,Complutense University (Spain), 3Department of Electrical and Computer Engineering,Tufts University

Chapters

  • 00:05Title
  • 01:20Microspheres Encoding
  • 02:42Preparation of Protein-capture Microspheres
  • 03:48Fiber-optic Microarray Assembly
  • 05:50Saliva Sample Analysis Using Microspheres Microarray
  • 07:10Results: Human Salivary Protein Analysis
  • 08:22Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述了使用多路复用基于微球的抗体阵列分析唾液蛋白的过程。单克隆抗体共价连接到荧光染料编码的4.5微米聚合物用碳二亚胺化学性微球。修改后的微球沉积在光纤的微孔用荧光夹心免疫测定蛋白水平在唾液。

Tags

MultiplexedFluorescentMicroarraySalivary ProteinAnalysisPolymer MicrospheresFiber-optic BundlesImmunoassaySuspension ArrayEncodingFluorescence MicroscopySandwich AssayBiotinylated Detection AntibodiesStreptavidinR-phycoerythrin

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