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Revealing the Cytoskeletal Organization of Invasive Cancer Cells in 3D
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JoVE Journal Medicine
Revealing the Cytoskeletal Organization of Invasive Cancer Cells in 3D

Revealing the Cytoskeletal Organization of Invasive Cancer Cells in 3D

DOI:
10.3791/50763-v

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11:09 min

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October 26, 2013

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, ,
1UMR 144 CNRS,Institut Curie

Chapters

  • 00:05Title
  • 01:48TAMRA-collagen I Labeling
  • 03:453D TAMRA-collagen Matrices with Embedded Single Cells
  • 05:133D TAMRA-collagen Matrices with Embedded Cell Spheroids
  • 06:343D Immunofluorescence Staining
  • 07:45Sample Imaging
  • 08:37Results: Representative 3D Immunofluorescent Images
  • 10:36Conclusion

Summary

Automatic Translation

Automatic Translation

This article presents a method to fluorescently label collagen that can be further used for both fix and live imaging of 3D cell cultures. We also provide an optimized protocol to visualize endogenous cytoskeletal proteins of cells cultured in 3D environments.

Tags

3D Cell CultureCell MigrationCancer CellsCytoskeletonCollagen I3D ImmunofluorescenceConfocal Microscopy

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