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在3D揭示侵入性癌症细胞骨架组织
JoVE Journal
Medicine
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JoVE Journal
Medicine
Revealing the Cytoskeletal Organization of Invasive Cancer Cells in 3D
Please note that all translations are automatically generated.
Click here for the English version.
在3D揭示侵入性癌症细胞骨架组织
DOI:
10.3791/50763-v
•
11:09 min
•
October 26, 2013
•
Sara Geraldo
,
Anthony Simon
,
Danijela M. Vignjevic
1
UMR 144 CNRS
,
Institut Curie
Chapters
00:05
Title
01:48
TAMRA-collagen I Labeling
03:45
3D TAMRA-collagen Matrices with Embedded Single Cells
05:13
3D TAMRA-collagen Matrices with Embedded Cell Spheroids
06:34
3D Immunofluorescence Staining
07:45
Sample Imaging
08:37
Results: Representative 3D Immunofluorescent Images
10:36
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文提出了一种荧光标记的胶原蛋白,可以进一步用于修复和实时成像的三维细胞培养的方法。我们还提供了一个可视化优化的协议在3D的环境中培养的细胞内源性细胞骨架蛋白。
Tags
3D Cell Culture
Cell Migration
Cancer Cells
Cytoskeleton
Collagen I
3D Immunofluorescence
Confocal Microscopy
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