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细胞器运输的培养<em>果蝇</em>细胞：S2细胞系和原代神经元。

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JoVE Journal Biology

Organelle Transport in Cultured Drosophila Cells: S2 Cell Line and Primary Neurons.

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细胞器运输的培养<em>果蝇</em>细胞：S2细胞系和原代神经元。

DOI:

10.3791/50838-v

•

10:08 min

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November 20, 2013

•

Wen Lu*¹, Urko del Castillo*^1,2, Vladimir I. Gelfand

¹Department of Cell and Molecular Biology,Feinberg School of Medicine, Northwestern University, ²IKERBASQUE,Basque Foundation for Science

Chapters

00:05Title
01:34Drosophila S2 Cells
03:19Drosophila Neuron Culture
07:47Visualized Microtuble Transport in Cultured S2 Cells and Neurons
09:42Conclusion

Summary

Automatic Translation

果蝇 S2细胞和培养的神经元是用于体内电机驱动的细胞器运输的成像大的系统。在这里，我们将详细介绍协议进行培养两种细胞类型，它们的成像和运输分析。

Tags

Drosophila S2 Cells Primary Neurons Organelle Transport Microtubules Live Imaging RNAi Cargo Transport Axonal Transport Cell Culture

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