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Transporte Organelle em Cultivadas
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Transporte Organelle em Cultivadas

Article DOI: 10.3791/50838-v • 10:08 min • November 20th, 2013
November 20th, 2013 •
*1, *1,2, 1
1Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, 2IKERBASQUE, Basque Foundation for Science
* These authors contributed equally

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Células Drosophila S2 e neurônios cultivados são grandes sistemas para a imagem latente de transporte organela motorizado in vivo. Aqui nós descrevemos protocolos detalhados para o cultivo de ambos os tipos de células, a sua imagem e análise de transporte.

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Biologia Celular Edição 81, Citoesqueleto células S2 cultura neurônio primário microtúbulos kinesin dineína microscopia de fluorescência imagens ao vivo
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