Journal
/
Biology
/
Organel Transport i dyrkede<em> Drosophila</em> Celler: S2 Cell Line og Primary neuroner.
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Organelle Transport in Cultured Drosophila Cells: S2 Cell Line and Primary Neurons.

Organel Transport i dyrkede<em> Drosophila</em> Celler: S2 Cell Line og Primary neuroner.

DOI:
10.3791/50838-v

•

10:08 min

•

November 20, 2013

•

, ,
1Department of Cell and Molecular Biology,Feinberg School of Medicine, Northwestern University, 2IKERBASQUE,Basque Foundation for Science

Chapters

  • 00:05Title
  • 01:34Drosophila S2 Cells
  • 03:19Drosophila Neuron Culture
  • 07:47Visualized Microtuble Transport in Cultured S2 Cells and Neurons
  • 09:42Conclusion

Summary

Automatic Translation

Automatic Translation

Drosophila S2-celler og dyrkede neuroner er gode systemer til billeddannelse af motordreven organel transport in vivo. Her beskriver vi detaljerede protokoller til dyrkning både celletyper, deres billedbehandling og analyse af transport.

Tags

DrosophilaS2 CellsPrimary NeuronsOrganelle TransportMicrotubulesLive ImagingRNAiCargo TransportAxonal TransportCell Culture

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Primary Neuronal Cultures from the Brains of Late Stage Drosophila Pupae

Live Imaging of Dense-core Vesicles in Primary Cultured Hippocampal Neurons

Preparation of Drosophila S2 cells for Light Microscopy

Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts

Primary Cell Cultures from Drosophila Gastrula Embryos

Isolering og Primær kultur Rotte levercellerne

Levende Imaging af Apoptotisk Cell Clearance under<em> Drosophila</em> Embryogenese

Imaging Local Ca<sup> 2+</sup> Signaler i dyrkede pattedyrceller

Generering af en "humaniseret"<em> Drosophila</em> S2 cellelinie Følsom over for Farmakologisk Inhibering af kinesin-5

Måling af glukoseoptagelse og reaktion på insulinstimulering i<em> In Vitro</em> Cultured Human Primary Myotubes

Read Article