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Die Cell-basierten L-Glutathion Schutz Assays zur Endozytose und Recycling von Plasmamembranproteinen Studieren
JoVE Journal
Biology
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JoVE Journal Biology
The Cell-based L-Glutathione Protection Assays to Study Endocytosis and Recycling of Plasma Membrane Proteins
DOI:

09:22 min

December 13, 2013

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Chapters

  • 00:05Title
  • 01:45Endocytic Assay: Biotinylation of the Apical Membrane Domain
  • 07:54Results: CFTR Endocytosis in CFBE41o- Cells
  • 08:49Conclusion

Summary

Automatic Translation

Membrane trafficking involves transport of proteins from the plasma membrane to the cell interior (i.e. endocytosis) followed by trafficking to lysosomes for degradation or to the plasma membrane for recycling. Methods described in this article are designed to study endocytosis and recycling of plasma membrane proteins.

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