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Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging
Journal (Biology)
Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging
Time-Lapse Video Microscopy for Assessment of EYFP-Parkin Aggregation as a Marker for Cellular Mitophagy
Journal (Biology)
Time-Lapse Video Microscopy for Assessment of EYFP-Parkin Aggregation as a Marker for Cellular Mitophagy
 
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Article DOI: 10.3791/51040 • 10:57 min • March 11th, 2014
March 11th, 2014 •
1, 1, 1, 1, 1
1Department of Biomedical Engineering, Washington University in St. Louis

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A abordagem lacuna vaselina cut-aberto é usado para obter baixos gravações de ruídos de correntes iônicas e gating de canais iônicos dependentes de voltagem expressos em oócitos de Xenopus com alta resolução de cinética de canais rápidos. Com modificações menores, fixador de tensão fluorometria pode ser acoplado com o protocolo do oócito corte aberto.

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Biologia do Desenvolvimento edição 85 braçadeira de tensão Cut-aberto de oócitos Voltagem Grampo Fluorometria os canais de sódio correntes iônicas,
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