Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Biology

A subscription to JoVE is required to view this content.
You will only be able to see the first 2 minutes.

The Xenopus Oocyte Cut-open Vaseline Gap Voltage-clamp Technique With Fluorometry
 

The Xenopus Oocyte Cut-open Vaseline Gap Voltage-clamp Technique With Fluorometry

Article DOI: 10.3791/51040 10:57 min
March 11th, 2014

Chapters

Summary March 11th, 2014

The cut-open Vaseline gap approach is used to obtain low noise recordings of ionic and gating currents from voltage-dependent ion channels expressed in Xenopus oocytes with high resolution of fast channel kinetics. With minor modification, voltage clamp fluorometry can be coupled to the cut-open oocyte protocol.

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter