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JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
La ligadura y punción cecal inducida por sepsis como un modelo para estudiar la autofagia en ratones
 
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La ligadura y punción cecal inducida por sepsis como un modelo para estudiar la autofagia en ratones

Article doi: 10.3791/51066
February 9th, 2014 •
1,3, 1, 2, 2, 1, 1
1Department of Medicine, Division of Pulmonary and Critical Care Medicine, Brigham and Women's Hospital, 2Department of Medicine, Renal Division, Brigham and Women's Hospital, Harvard Medical School, 3First Department of Critical Care Medicine and Pulmonary Services, University of Athens Medical School, Evangelismos Hospital, Athens, Greece

Summary
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February 9th, 2014

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La sepsis experimental puede ser inducida en ratones usando el método (CLP) ligadura y punción cecal. Los protocolos actuales para evaluar la autofagia in vivo en el contexto de la sepsis inducida por CLP se presentan aquí: Un protocolo para la medición de la autofagia utilizando ratones (GFP)-LC3, y de un protocolo para la medición de la formación de autofagosoma por microscopía electrónica.

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