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Design og drift av en kontinuerlig<sup> 13</sup> C og<sup> 15</sup> N Merking Chamber for Uniform eller Differential, Metabolic and Structural, Plant Isotop Merking
JoVE Journal
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Design and Operation of a Continuous 13C and 15N Labeling Chamber for Uniform or Differential, Metabolic and Structural, Plant Isotope Labeling
DOI:

10:16 min

January 16, 2014

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Chapters

  • 00:05Title
  • 01:26Chamber Construction
  • 02:37Temperature, Humidity, and CO2 Controls
  • 04:51Irrigation System and Potting Plants
  • 06:23Fertilization with a 15N-labeled Fertilizer
  • 07:22Harvest
  • 08:14Results: Successful Incorporation of 13C and 15N into Plant Tissue
  • 09:51Conclusion

Summary

Automatic Translation

Denne metoden forklarer hvordan du skal bygge og drive et kontinuerlig 13 C og 15 N isotop merking kammer for uniform eller differensial plantevev merking. Representative resultater fra metabolske og strukturelle merking av Andropogon gerardii blir diskutert.

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