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JoVE Journal Chemistry
Bestemmelse af isbindende fly af frostvæskeproteiner ved fluorescens-baserede Ice Plane Affinitet
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Bestemmelse af isbindende fly af frostvæskeproteiner ved fluorescens-baserede Ice Plane Affinitet

Article DOI: 10.3791/51185-v • 08:46 min • January 15th, 2014
January 15th, 2014 •
1, 2, 3, 3, 4, 1
1Department of Biomedical and Molecular Sciences, Queen's University, 2National Institute of Neurological Disorders and Stroke, Porter Neuroscience Research Center, 3Research Institute of Genome-Based Biofactory, National Institute of Advanced Industrial Science and Technology, 4The Robert H. Smith Faculty of Agriculture, Food and Environment, Institute of Biochemistry, Food Science, and Nutrition, The Hebrew University of Jerusalem

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Frostvæskeproteiner (AFP'er) binder sig til specifikke isplaner for at forhindre eller bremse isvækst. Fluorescens-baserede isplan affinitet (FIPA) analyse er en ændring af den oprindelige is-ætsning metode til bestemmelse af AFP-bundet is planer. AFP'er er fluorescerende mærket, indarbejdet i makroskopiske enkeltiskrystaller og visualiseret under UV-lys.

Tags

Kemi Materialer Life Sciences Optik frostvæske proteiner Ice adsorption Fluorescerende mærkning Ice gitter fly isbindende proteiner Enkelt is krystal
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