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JoVE Journal Bioengineering
Characterization of Complex Systems Using the Design of Experiments Approach
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Characterization of Complex Systems Using the Design of Experiments Approach: Transient Protein Expression in Tobacco as a Case Study

Article DOI: 10.3791/51216-v • 20:24 min • January 31st, 2014
January 31st, 2014 •
1, 1,2
1Institute for Molecular Biotechnology, RWTH Aachen University, 2Institute for Molecular Biology and Applied Ecology, Fraunhofer Gesellschaft

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We describe a design of experiments approach that can be used to determine and model the influence of transgene regulatory elements, plant growth and development parameters, and incubation conditions on the transient expression of monoclonal antibodies and reporter proteins in plants.

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Complex Systems Design Of Experiments Transient Protein Expression Tobacco Case Study Biopharmaceuticals Scalability Safety Regulatory Concerns Good Manufacturing Practice Expression Construct Plant Growth And Development Incubation Conditions Variability Of Expression Anti-HIV Monoclonal Antibody Fluorescent Marker Protein Rationale For Selecting Properties Potential Limitations Knowledge-based Parameter Selection Complexity Reduction Optimal Experiment Combinations Step-wise Design Augmentation
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