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JoVE Journal
Biology

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前体mRNA底物的3'末端切割:用无细胞体系中的RNA加工反应分析
 
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前体mRNA底物的3'末端切割:用无细胞体系中的RNA加工反应分析

Article DOI: 10.3791/51309-v 09:16 min May 3rd, 2014
May 3rd, 2014

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Summary

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Click here for the English version.

RNA聚合酶II合成延伸超过所述成熟mRNA的3'末端的前体RNA。的成熟RNA的末端cotranscriptionally生成的,在由RNA序列决定的一个部位,通过裂解复合物的内切核酸酶的活性。在这里,我们详细研究裂解反应在体外的方法。

Tags

传染病,87期,卵裂,多聚腺苷酸化,mRNA加工,核提取物,3'加工复杂
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