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基于海拉细胞自由表达系统中的表达<em>疟原虫</em>棒状体蛋白

JoVE Journal
Biology

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JoVE Journal Biology

HeLa Based Cell Free Expression Systems for Expression of Plasmodium Rhoptry Proteins

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基于海拉细胞自由表达系统中的表达<em>疟原虫</em>棒状体蛋白

DOI:

10.3791/52772-v

•

09:03 min

•

June 10, 2015

•

Raghavendra Yadavalli, Tobili Sam-Yellowe

¹Department of Biological, Geological, and Environmental Sciences,Cleveland State University

Chapters

00:05Title
02:12Protein Expression Using In Vitro Human Cell Free Expression System
03:21Purification of Expressed Recombinant Proteins
05:05Western Blotting
06:52Results: Purple Color Band on Nitrocellulose Paper During Developing Step Confirms the Successful Expression of Proteins
08:11Conclusion

Summary

Automatic Translation

基于细胞系统疟疾蛋白的表达仍然具有挑战性。我们证明两步并一步IVT（体外翻译）无细胞表达系统从HeLa细胞表达的重组疟疾蛋白质棒状体。我们使用的Ni树脂基于亲和性的纯化系统纯化棒状体蛋白质。

Tags

HeLa Cell-free Expression Plasmodium Proteins Malaria Vaccine Candidates Cell-free Expression Systems Protein Expression In Vitro Human Cell-free Expression PF3D7 0114100 PF3D7 0925900 PF3D7 1361800 Protein Yield Affinity Purification

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