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Protein-protein interaktioner visualiseras genom Bimolecular Fluorescens Komplettering i tobaksprotoplaster och Löv
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Protein-protein interaktioner visualiseras genom Bimolecular Fluorescens Komplettering i tobaksprotoplaster och Löv

Article DOI: 10.3791/51327-v • 11:10 min • March 9th, 2014
March 9th, 2014 •
1, 1
1Department Biologie I, Botanik, Ludwig-Maximilians-Universität, München

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Bildning av protein-komplex in vivo kan visualiseras genom bimolekylära fluorescens komplementering. Interaktionspartner är fuserade till komplementära delar av fluorescerande taggar och uttrycktes transient i tobaksblad, vilket resulterar i en rekonstituerad fluorescerande signal vid närhet av de två proteinerna.

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Växtbiologi Tetratricopeptide upprepa domän förkläde kloroplaster endoplasmatiska retiklet HSP90 Toc komplex Sec translocon BiFC
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