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March 09, 2014
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Summary
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A formação de complexos de proteína in vivo, podem ser visualizados por fluorescência bimolecular complementação. Parceiros de interacção são fundidas com as peças complementares de marcas fluorescentes e transitoriamente expressa em folhas de tabaco, resultando num sinal fluorescente reconstituído na estreita proximidade das duas proteínas.
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Cite this Article
Schweiger, R., Schwenkert, S. Protein-protein Interactions Visualized by Bimolecular Fluorescence Complementation in Tobacco Protoplasts and Leaves. J. Vis. Exp. (85), e51327, doi:10.3791/51327 (2014).
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