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小鼠胚胎皮肤和Live成像黑素细胞迁移的体外培养
JoVE Journal
Biology
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JoVE Journal Biology
Ex vivo Culture of Mouse Embryonic Skin and Live-imaging of Melanoblast Migration
DOI:

08:29 min

May 19, 2014

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Chapters

  • 00:05Title
  • 01:26Dissection of Embryonic Skin
  • 02:54Mounting the Skin
  • 05:46Live Imaging
  • 06:49Live Imaging of Developing Melanoblasts
  • 07:59Conclusion

Summary

Automatic Translation

我们描述了小鼠胚胎皮肤的解剖和体外培养。培养系统保持一个气 - 液界面在整个组织表面,并允许成像于倒置显微镜。黑素细胞中,显影皮肤的成分,被荧光标记的允许使用共聚焦显微镜观察其行为。

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