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Journal
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Biology
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小鼠胚胎皮肤和Live成像黑素细胞迁移的
体外
培养
JoVE Journal
Biology
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JoVE Journal
Biology
Ex vivo
Culture of Mouse Embryonic Skin and Live-imaging of Melanoblast Migration
Please note that all translations are automatically generated.
Click here for the English version.
小鼠胚胎皮肤和Live成像黑素细胞迁移的
体外
培养
DOI:
10.3791/51352-v
•
08:29 min
•
May 19, 2014
•
Richard L. Mort
,
Margaret Keighren
,
Leonard Hay
,
Ian J. Jackson
1
MRC Human Genetics Unit, MRC IGMM, Western General Hospital
,
University of Edinburgh
Chapters
00:05
Title
01:26
Dissection of Embryonic Skin
02:54
Mounting the Skin
05:46
Live Imaging
06:49
Live Imaging of Developing Melanoblasts
07:59
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了小鼠胚胎皮肤的解剖和
体外
培养。培养系统保持一个气 - 液界面在整个组织表面,并允许成像于倒置显微镜。黑素细胞中,显影皮肤的成分,被荧光标记的允许使用共聚焦显微镜观察其行为。
Tags
Ex Vivo Culture
Mouse Embryonic Skin
Live-imaging
Melanoblast Migration
Neural Crest Cell Migration
Air-liquid Interface
Confocal Microscopy
Melanoblast-specific Cre-recombinase
R26YFPR Reporter
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