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En effektiv metode til kvantitative, Single-celle Analyse af Kromatin Ændring og nuklear Arkitektur i Whole-Mount frøanlæg i
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En effektiv metode til kvantitative, Single-celle Analyse af Kromatin Ændring og nuklear Arkitektur i Whole-Mount frøanlæg i

Article DOI: 10.3791/51530-v • 09:33 min • June 19th, 2014
June 19th, 2014 •
1, 2, 1
1Institute of Plant Biology and Zürich-Basel Plant Science Center, University of Zürich, 2Institut de Recherche pour le Développement (UMR 232), Centre National de la Recherche Scientifique (ERL 5300), Université de Montpellier II

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Vi giver her en effektiv og pålidelig protokol til immunfarvning, Fluorescens in situ hybridisering, DNA-farvning efterfulgt af kvantitativ, høj opløsning billeddannelse i hel-mount Arabidopsis thaliana frøanlæg. Denne metode blev anvendt med succes til at analysere kromatin ændringer og nuklear arkitektur.

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Plantebiologi , Ovule kromatin modifikation nuklear arkitektur immunfarvning Fluorescens FISH DNA farvning heterochromatin
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