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细胞质DNA的检测途径的刺激<em>在体外</em>和<em>体内</em
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Biology
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JoVE Journal
Biology
Stimulation of Cytoplasmic DNA Sensing Pathways
In Vitro
and
In Vivo
Please note that all translations are automatically generated.
Click here for the English version.
细胞质DNA的检测途径的刺激<em>在体外</em>和<em>体内</em
DOI:
10.3791/51593-v
•
11:44 min
•
September 18, 2014
•
Chih Hung Ku
,
Brian J. Ferguson
1
Department of Pathology
,
University of Cambridge
Chapters
00:05
Title
01:17
Concatemerization of Immuno-stimulatory DNA Sequences
02:43
DNA Purification and Analysis
05:55
Transfection of dsDNA Concatemers
In Vitro
06:47
Transfection of dsDNA Concatemers
In Vivo
08:29
Processing of DNA Stimulated Samples and Quantitative PCR Analysis
10:32
Results: Concatermerized DNA Stimulate an Innate Immune Response
In Vitro
and
In Vivo
11:14
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议的目的是要使用的最佳方法用于刺激细胞质DNA传感途径在细胞和
体内
。这是通过平末端连接时改善长时间,双链DNA的生成来实现的。细胞或小鼠用脂质转染试剂转染。
Tags
DNA Sensing Pathways
Cytoplasmic DNA
Double-stranded DNA
Oligonucleotides
Concatemerization
Polyethylene Glycol
Innate Immune Response
In Vitro
In Vivo
Transfection
Intradermal Injection
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