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Collection and Extraction of Saliva DNA for Next Generation Sequencing
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JoVE Journal Medicine
Collection and Extraction of Saliva DNA for Next Generation Sequencing

Collection and Extraction of Saliva DNA for Next Generation Sequencing

DOI:
10.3791/51697-v

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06:58 min

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August 27, 2014

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, , , ,
1Battelle Center for Mathematical Medicine,The Research Institute at Nationwide Children’s Hospital, 2The Interdisciplinary Graduate Program in Biophysics,The Ohio State University, 3Department of Pediatrics,The Ohio State University

Chapters

  • 00:05Title
  • 01:01Saliva Collection and Storage
  • 01:46Initial Preparations, Cell Lysis and RNA, Protein and Lipid Removal
  • 03:46Isolation and Purification of Genomic DNA
  • 05:43Genomic DNA Characterization
  • 06:35Conclusion

Summary

Automatic Translation

Automatic Translation

DNA extraction from saliva can provide a readily available source of high molecular weight DNA, with little to no degradation/fragmentation. This protocol provides optimized parameters for saliva collection/storage and DNA extraction to be of sufficient quality and quantity for downstream DNA assays with high quality requirements.

Tags

Keywords: DNA ExtractionSalivaNext-generation SequencingDNA CollectionDNA QualityDNA QuantityDNA AssaysMicroarray Genotyping

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