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Biology

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Zink-vinger Nuclease Enhanced gentargeting in menselijke embryonale stamcellen
 
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Zink-vinger Nuclease Enhanced gentargeting in menselijke embryonale stamcellen

Article DOI: 10.3791/51764
August 23rd, 2014

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Summary August 23rd, 2014

Please note that all translations are automatically generated.

Click here for the English version.

Reporter cellijnen bieden een middel om te visualiseren, te volgen en te isoleren cellen van belang zijn van de heterogene populatie. Echter, gen targeting met gebruikelijke homologe recombinatie in humane embryonale stamcellen is inefficiënt. Hierin beschrijven we richten CNS middenhersenen specifieke transcriptiefactor PITX3 locus met EGFP met zink-vinger nuclease verbeterde homologe recombinatie.

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