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In Vivo Microinjection and Electroporation of Mouse Testis
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In Vivo Microinjection and Electroporation of Mouse Testis

Article DOI: 10.3791/51802-v • 08:39 min • August 23rd, 2014
August 23rd, 2014 •
1, 1, 1
1Institute of Reproductive Biology, Leibniz Institute for Farm Animal Biology (FBN)

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This article describes microinjection and electroporation of mouse testis in vivo as a transfection technique for testicular mouse cells to study unique processes of spermatogenesis. The presented protocol involves steps of glass capillary preparation, microinjection via the efferent duct, and transfection by electroporation.

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Microinjection Electroporation Mouse Testis Transfection Technique Gene Transfer Reporter Plasmid Constructs EGFP DsRed2 CMV Promoter Anaesthetized Animal Efferent Duct Rete Testis Seminiferous Tubules Electroporation DNA Solution
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