A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Department of Nanomedicine,Houston Methodist Research Institute, 2MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering,Sun Yat-sen University, 3Pediatrics Department of Union Hospital,Huazhong University of Science and Technology, 4CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety,National Center for Nanoscience & Technology of China, 5Department of Medicine,Weill Cornell Medical College, 6Department of Cell and Developmental Biology,Weill Cornell Medical College
Chapters
- 00:05Title
- 00:58PCPS Particle Preparation
- 03:46PCPS Particle Characterization
- 04:33Loading of siRNA into PCPS Particles and Optimization of siRNA/PCPS Particle Ratio
- 05:31Release of siRNA from PCPS Particles
- 06:16Results: Characterization of Porous Silicon Microparticles for siRNA Delivery
- 08:02Conclusion
Entrega continua a ser o principal desafio para a aplicação terapêutica de RNA de interferência pequeno (siRNA). Este protocolo envolve a utilização de uma plataforma de entrega de siRNA multifuncional e biocompativel, que consiste de arginina e polietilenoimina enxertado micropartículas de silício poroso.
