Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Electrophoretic Mobility Shift Assay (EMSA) für…
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

JoVE Journal Biology
Electrophoretic Mobility Shift Assay (EMSA) für das Studium von RNA-Protein-Wechselwirkungen
Large Insert Environmental Genomic Library Production
Journal (Biology)
Large Insert Environmental Genomic Library Production
In Vitro Aggregation Assays Using Hyperphosphorylated Tau Protein
Journal (Biology)
In Vitro Aggregation Assays Using Hyperphosphorylated Tau Protein
 
Click here for the English version

Electrophoretic Mobility Shift Assay (EMSA) für das Studium von RNA-Protein-Wechselwirkungen: Die IRE / IRP Beispiel

Article DOI: 10.3791/52230 • 12:44 min • December 3rd, 2014
December 3rd, 2014 •
1,2, 1,2, 1,2
1Lady Davis Institute for Medical Research, Jewish General Hospital, 2Department of Medicine, McGill University

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Hier präsentieren wir ein Protokoll zur RNA / Protein-Interaktionen zu untersuchen. Elektrophoretische Mobilitäts-Shift-Assay (EMSA) auf der differentielle Migration RNA / Protein-Komplexe und freie RNA während nativer Gelelektrophorese. Durch Verwendung eines radioaktiv markierten RNA-Sonde kann RNA / Protein-Komplexe durch Autoradiographie visualisiert werden.

Tags

Biochemie RNA-Metabolismus mRNA-Translation post-transkriptionale Genregulation mRNA-Stabilität IRE IRP1 irp2 Eisenstoffwechsels Ferritin Transferrin-Rezeptor
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter