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Chapters
- 00:05Title
- 01:28Preparation of Protein Extracts from Cultured Cells
- 03:05Preparation of Protein Extracts from Mouse Liver and Spleen
- 04:56Synthesis of Radiolabeled IRE-containing RNA Probe
- 05:55Purification of Radiolabeled IRE-containing RNA Probe
- 07:48Electrophoretic Mobility Shift Assay (EMSA)
- 10:27Results: The Iron-dependence of IRE/IRP Interactions
- 12:17Conclusion
Her præsenterer vi en protokol til at analysere RNA / protein-interaktioner. Den elektroforetiske mobilitet (EMSA) er baseret på forskellen migration af RNA / protein-komplekser og fri RNA under nativ gelelektroforese. Ved anvendelse af en radioaktivt mærket RNA-probe, kan RNA / protein komplekser visualiseret ved autoradiografi.
