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Extraction of Non-Protein Amino Acids from Cyanobacteria for Liquid Chromatography-Tandem Mass Spectrometry Analysis
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Chemistry
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JoVE Journal Chemistry
Extraction of Non-Protein Amino Acids from Cyanobacteria for Liquid Chromatography-Tandem Mass Spectrometry Analysis

Extraction of Non-Protein Amino Acids from Cyanobacteria for Liquid Chromatography-Tandem Mass Spectrometry Analysis

DOI:
10.3791/63779-v

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08:14 min

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December 09, 2022

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, , , ,
1Hyphenated Mass Spectrometry Laboratory (HyMaS),University of Technology Sydney, 2School of Life Sciences,University of Technology Sydney

Chapters

  • 00:05Introduction
  • 00:44Raw Sample Preparation
  • 01:56Cell Lysing and Fractionation of Free Non-Protein Amino Acids (NPAAs)
  • 03:36Fractionation of the Protein-Bound Non-Protein Amino Acids (NPAAs)
  • 06:11Sample Filtering
  • 06:35Results: Extraction of NPAAs from Biological Matrices via TCA Protein Precipitation and Acid Hydrolysis
  • 07:15Conclusion

Summary

Automatic Translation

Automatic Translation

The present protocol describes the extraction of non-protein amino acids from biological matrices via trichloroacetic acid (TCA) protein precipitation and acid hydrolysis before analysis using liquid chromatography-tandem mass spectrometry.

Tags

Keywords: Non-protein Amino AcidsCyanobacteriaLiquid Chromatography-tandem Mass SpectrometryBMAAEG24-DABExtractionFreeze DryingSonicationCentrifugationTrichloroacetic Acid

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