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Multi-Faceted Mass Spectrometric Investigation of Neuropeptides in Callinectes sapidus
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Multi-Faceted Mass Spectrometric Investigation of Neuropeptides in Callinectes sapidus

Article DOI: 10.3791/63322-v • 09:22 min • May 31st, 2022
May 31st, 2022 •
*1, *1, 1,2
1Department of Chemistry, University of Wisconsin-Madison, 2School of Pharmacy, University of Wisconsin-Madison
* These authors contributed equally

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Summary
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Mass spectrometric characterization of neuropeptides provides sequence, quantitation, and localization information. This optimized workflow is not only useful for neuropeptide studies, but also other endogenous peptides. The protocols provided here describe sample preparation, MS acquisition, MS analysis, and database generation of neuropeptides using LC-ESI-MS, MALDI-MS spotting, and MALDI-MS imaging.

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Neuropeptides Mass Spectrometry Endogenous Peptides Peptide Analysis Neuropeptide Extraction Quantitation Localization Peptide Therapeutics Neuropeptide Biomarkers Brain Tissue Acidified Methanol Ultrasonic Homogenizer Centrifuge Vacuum Concentrator Desalting
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