Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo: A Model of Single Vessel Stroke

The overall goal of this procedure is to use a semi invasive optical microscopy approach to induce a rose bele photo thrombotic clot in the somatosensory cortex of a mouse. This is accomplished by anesthetizing the mouse and preparing a thin skull cranial window for imaging. Next, the rose bengal is injected and a laser is used to induce a photo thrombosis.

Ultimately, the effects of the clot made by the rose Bengal photo thrombosis can then be immediately analyzed after recovery from anesthesia. The main advantage of this technique is that it allows in vivo cellular responses to be seen immediately after rose bengal photo thrombosis is induced. Individuals new to this technique may struggle with creating the thin skull cranial window because it is difficult and time consuming.