A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 00:49Synthesis of Magnetic Nanoparticles (MNPs)
- 03:07Chemical Modification of Magnetic Nanoparticles
- 05:53Magnetic Particle Size Analysis Using DLS
- 06:46Testing Efficiency of DNA Isolation Using Quantitative PCR
- 07:58Magnetic Particle Zeta Potential Analysis Using DLS
- 08:37Results: Zeta Potential of Representative Magnetic Particles Over Time in Presence and Absence of DNA
- 09:57Conclusion
This protocol describes the synthesis of magnetic particles and evaluation of their DNA-binding properties via dynamic and electrophoretic light scattering. This method focuses on monitoring changes in particle size, their polydispersity and zeta potential of particle surface which play major role in binding of materials such as DNA.
