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使用Tomoauto:一个协议,用于高通量自动化低温电子断层扫描
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Bioengineering
Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography
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使用Tomoauto:一个协议,用于高通量自动化低温电子断层扫描
DOI:
10.3791/53608-v
•
11:33 min
•
January 30, 2016
•
Dustin R. Morado
,
Bo Hu
,
Jun Liu
1
Department of Pathology and Laboratory Medicine
,
University of Texas Health Science Center at Houston
Chapters
00:05
Title
01:03
Low-magnification Map Collection
03:22
Tilt-series Collection
05:30
High-throughput Automated Tilt-series Processing and Reconstruction Using Tomoauto
09:08
Results: Tomogram and Sub-tomogram Views of S. flexneri
10:31
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们提出如何利用高通量低温电子断层扫描,以确定高分辨率
的
分子机器的结构
原位
的协议。该协议允许大量数据要处理,避免了常见的瓶颈,并减少停机时间资源,从而允许用户集中在重要的生物的问题。
Tags
Cryo-electron Tomography
High-throughput
Automated
Data Processing
Tilt-series
3D Reconstruction
Shigella Flexneri
Electron Microscopy
Grid Preparation
Low-magnification Mapping
Stage Positioning
Montage Setup
Navigator Acquisition
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