Journal
/
/
使用Tomoauto:一个协议,用于高通量自动化低温电子断层扫描
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography
DOI:

11:33 min

January 30, 2016

, ,

Chapters

  • 00:05Title
  • 01:03Low-magnification Map Collection
  • 03:22Tilt-series Collection
  • 05:30High-throughput Automated Tilt-series Processing and Reconstruction Using Tomoauto
  • 09:08Results: Tomogram and Sub-tomogram Views of S. flexneri
  • 10:31Conclusion

Summary

Automatic Translation

我们提出如何利用高通量低温电子断层扫描,以确定高分辨率分子机器的结构原位的协议。该协议允许大量数据要处理,避免了常见的瓶颈,并减少停机时间资源,从而允许用户集中在重要的生物的问题。

Related Videos

Read Article