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Immunology and Infection

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JoVE Journal Immunology and Infection
A Rapid Strategy for the Isolation of New Faustoviruses from Environmental Samples Using Vermamoeba vermiformis
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A Rapid Strategy for the Isolation of New Faustoviruses from Environmental Samples Using Vermamoeba vermiformis

Article DOI: 10.3791/54104-v • 09:27 min • June 4th, 2016
June 4th, 2016 •
1, 1, 1,2, 1,2
1Faculty of Medicine and Pharmacy, Research Unit for Infectious and Tropical Emerging Diseases, Aix Marseille University, 2Pole of Infectious and Tropical Diseases, Clinical and Biological Sector, Federation of Bacteriology-Hygiene Virology, University Hospital Institute Mediterranean Infection

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Summary
Automatic Translation

We describe here the latest advances in viral isolation for the characterization of new genotypes of Faustovirus, a new asfarvirus-related lineage of giant viruses. This protocol can be applied to the high throughput isolation of viruses, especially giant viruses infecting amoeba.

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Isolation Strategy Faustoviruses Environmental Samples Vermamoeba Vermiformis Pan-genome Viral Family Ecosystems Pathogenicity Virology Field Giant Viruses Antibiotic And Antifungal Mixture Starvation Medium Floss-cytometry Lysis Detection Dirty Water Samples Host Cell Preparation V.vermiformis Protease Peptone Yeast Extract Glucose (PYG) Medium Incubation Temperature Centrifugation Counting Slides Concentration Of Cells Per Milliliter Harvest Cells Sterile Pages Amoebal Saline (PAS) Antibiotic
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