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Udtømning af Mouse Celler fra Humane tumorxenoplantater Markant Forbedrer Downstream Analyse af Target Cells
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal Medicine
Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells
DOI:

07:10 min

July 29, 2016

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Chapters

  • 00:05Title
  • 00:40Tumor Dissociation
  • 02:06Magnetic Cell Separation Mouse Cell Depletion
  • 04:01Representative Results: Downstream Mouse Cell-free Human Tumor Cell Analyses
  • 05:58Conclusion

Summary

Automatic Translation

Humane tumor-xenotransplantater er vaskulariseret og infiltreret af celler af museoprindelse under vækstfasen in vivo. For at omgå bias forårsaget af disse kontaminerende celler under nedstrøms analyse, udviklede vi en fremgangsmåde muliggør omfattende udtømning af alle museceller ved magnetisk cellesortering.

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